5-FAM(5-羧基熒光素)的用途以及儲(chǔ)存條件

2020-05-08 13:56:24      點(diǎn)擊:

5-FAM ;5-羧基熒光素

分子式:C21H12O7    分子量:376.32
激發(fā)波長(zhǎng)(EX): 492nm
發(fā)射波長(zhǎng)(EM): 518nm
溶解性:溶于DMSO
用途:5-FAM是一個(gè)純化的單一羧基異構(gòu)體。它是最普遍的用來(lái)標(biāo)記肽,蛋白質(zhì)及核酸的綠色熒光試劑之一。它主要用來(lái)氬激光器488nm處被激發(fā)的一系列綠色熒光肽。同時(shí)也可用于配制各種小熒光分子。
5-FAM is the purified single isomer of carboxyfluorescein. It is one of the most popular green fluorescent reagents used for labeling peptides, proteins and nucleotides. It has been predominantly used to develop a variety of green fluorescent peptides that can be excited with the 488 nm line of the Ar laser. It has also been used to prepare various small fluorescent molecules.


5-羧基熒光素是一種可用作酸堿指示劑的熒光染料,5-羧基熒光素具有極強(qiáng)的濃度依賴性并且可以顯示較強(qiáng)的熒光,可通過(guò)羧基和氨基的反應(yīng)標(biāo)記蛋白質(zhì),核甘酸和多肽。5-FAM
存儲(chǔ)條件(Storage conditions): 2-8°C避光干燥保存。
Storage: 2-8°C desiccated and protected from light.
最大激發(fā)波長(zhǎng)(Excitation maximum): 494nm
最大發(fā)射波長(zhǎng)(Emission maximum): 522nm
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